with 24 users it's acceptable to run the hubs off the switch and have 3 collision domains. i doubt your users will saturate this network but if you find any slowdown or if your users transfer large files, drop a sniffer on the network before and after you put the switch in and look at your utilization and collisions. it'd be a good idea to establish a baseline on your network utilization..

remember that when your on a 100Mb hub, the highest throughput your going to get is about 30-40 Mb/s for the entire collision domain but when your on a switch you can get 100Mb/s for each point to point connection. for example, if you have 10 machines and each user is connected to only one other user for a single instance, you can get 500Mb/s throughput on that switch while running in half duplex and 1Gb/s throughput in full duplex mode.

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